recombinant mouse il 12 protein  (R&D Systems)

Journal: Nature Communications

Article Title: In situ self-assembled cell reservoir hydrogel for maneuvering multistage radioimmunotherapy

doi: 10.1038/s41467-026-68490-5

Figure Lengend Snippet: a Upon intratumoral injection of the hydrogel precursors, BaO 2 reacts with hydrogen ions within the TME to produce Ba 2+ and H 2 O 2 in situ. Subsequently, the generated H 2 O 2 can be catalyzed by the CAT on RBC to supply oxygen for aerobic RT to augment sequential aCTLA-4/IL-12 release. b In the primary stage of RIT, aCTLA-4 is introduced to alleviate the tumor immunosuppression, thus primarily inducing DC homing and T cell activation. Afterward, IL-12 can initiate the immunoactivation in the relay stage to instigate the production of IFN-γ from T/NK cells for boosted antitumor immune responses.

Article Snippet: Horseradish peroxidase (HRP) (Cat# P8020), BCA protein assay kit (Cat# PC0020), live/dead cell double stain kit (Cat #CA1630), and red blood cell lysis buffer (Cat #R1010) were purchased from Beijing Solarbio Science & Technology Co., Ltd. Recombinant Mouse IL-12 (Cat# CM39) was purchased from Novoprotein.

Techniques: Injection, In Situ, Generated, Activation Assay

Journal: Nature Communications

Article Title: In situ self-assembled cell reservoir hydrogel for maneuvering multistage radioimmunotherapy

doi: 10.1038/s41467-026-68490-5

Figure Lengend Snippet: a Schematic showing the synthesis process of IL/aC@RBC. b SDS-PAGE protein analysis of nRBC, RBC, IL/aC@RBC, IL-12, and aCTLA-4. Marker indicates the molecular weight in kilodaltons (kDa). c Representative CLSM images of IL/aC@RBC. RBCM, IL-12, and aCTLA-4 were respectively labeled with DiI (red), AMCA (blue), and FITC (green). Scale bar, 2 μm. d Fluorescence colocalization spectra of RBCM-DiI, IL-12-AMCA, and aCTLA-4-FITC in IL/aC@RBC, as shown in ( c ). e SEM images of nRBC and IL/aC@RBC. Scale bar, 2 μm. f Relative enzyme activity analysis of CAT in nRBC, RBC, IL/aC@RBC and RBCM. g In vitro O 2 production from nRBC and IL/aC@RBC. h IFN-γ concentrations secreted by murine splenocytes subjected to IL-12 and IL@RBC. i High-resolution TEM images of BaO 2 . Scale bars, 100 nm and 20 nm. j XPS spectrum of BaO 2 . k Release profiles of Ba 2+ from BaO 2 at different pH values. l H 2 O 2 generation from BaO 2 at different pH values. Experiments in ( b , c , e , i ) were independently repeated three times with comparable results. Data in ( f , g , h , k , l ) were presented as mean ± SD. n = 3 independent experiments. Statistical significance was determined using two-way ANOVA ( k ) and two-sided unpaired student’s t -test ( l ). Source data are provided as a Source Data file.

Article Snippet: Horseradish peroxidase (HRP) (Cat# P8020), BCA protein assay kit (Cat# PC0020), live/dead cell double stain kit (Cat #CA1630), and red blood cell lysis buffer (Cat #R1010) were purchased from Beijing Solarbio Science & Technology Co., Ltd. Recombinant Mouse IL-12 (Cat# CM39) was purchased from Novoprotein.

Techniques: SDS Page, Marker, Molecular Weight, Labeling, Fluorescence, Activity Assay, In Vitro

Journal: Nature Communications

Article Title: In situ self-assembled cell reservoir hydrogel for maneuvering multistage radioimmunotherapy

doi: 10.1038/s41467-026-68490-5

Figure Lengend Snippet: a Scheme for the in situ synthetic route and biodegradation performance of IL/aC@RBAH. b Photographs of IL/aC@RBAH following the pH adjustment. c Changes of G’ and G” of IL/aC@RBAH at pH 7.4 and pH 6.5. d Strain amplitude sweeps of IL/aC@RBAH with different feeding concentrations of BaO 2 at a frequency of 1 Hz. e Dynamic oscillatory frequency sweeps of IL/aC@RBAH with different feeding concentrations of BaO 2 at a constant strain of 2.5%. f Representative CLSM images of IL/aC@RBAH. RBCM, IL-12, and aCTLA-4 were respectively labeled with DiI (red), AMCA (blue), and FITC (green). Scale bar, 50 μm. g Cryo-SEM image presenting the microstructure of IL/aC@RBAH. Scale bar, 2.5 μm. h Degradation behavior of IL/aC@RBAH within 14 days. i Cumulative release profiles of aCTLA-4 and IL-12 from IL/aC@RBAH at pH 7.4 and pH 6.5 within 48 h. j Cumulative release profile of Ba 2+ from IL/aC@RBAH within 48 h. k Representative CLSM images for live/dead staining of 4T1 cells subjected to IL/aC@RBAH for 24 h. Green, Calcein-AM, live cells. Red, PI, dead cells. Experiments in ( b , f , g , k ) were independently repeated three times with comparable results. Data in ( h – j ) were presented as mean ± SD. n = 3 independent experiments. Statistical significance was determined using two-way ANOVA ( i ). Source data are provided as a Source Data file.

Article Snippet: Horseradish peroxidase (HRP) (Cat# P8020), BCA protein assay kit (Cat# PC0020), live/dead cell double stain kit (Cat #CA1630), and red blood cell lysis buffer (Cat #R1010) were purchased from Beijing Solarbio Science & Technology Co., Ltd. Recombinant Mouse IL-12 (Cat# CM39) was purchased from Novoprotein.

Techniques: In Situ, Labeling, Staining

Journal: Nature Communications

Article Title: In situ self-assembled cell reservoir hydrogel for maneuvering multistage radioimmunotherapy

doi: 10.1038/s41467-026-68490-5

Figure Lengend Snippet: a Photographs showing the in vivo degradation behavior of subcutaneous IL/aC@RBAH on different days. b , c Whole-animal in vivo fluorescence imaging ( b ) and corresponding fluorescence quantification ( c ) of orthotopic 4T1 tumor-bearing mice after intratumoral injection of IL-Cp&aC, IL-Cp/aC@RBC, and IL-Cp/aC@RBAH. d Concentrations of aCTLA-4 in serum of 4T1 tumor-bearing mice after intratumoral injection of saline, aCTLA-4, and IL/aC@RBAH. e Concentrations of IL-12 in serum of 4T1 tumor-bearing mice after intratumoral injection of saline, IL-12, and IL/aC@RBAH. f – i Detection of liver function indexes including ALT ( f ) and AST ( g ), and kidney function indexes, including BUN ( h ) and CREA ( i ) in 4T1 tumor-bearing mice after intratumoral injection of saline, IL&aC, RBAH, and IL/aC@RBAH. The normal range of liver function and kidney function indexes was marked by dash lines. j Representative PA images and corresponding quantification of sO 2 levels in 4T1 tumors before and after injection of IL/aC@RBC and IL/aC@RB. k Representative PA images and corresponding quantification of sO 2 levels in 4T1 tumors at different time points after injection of IL/aC@RB and IL/aC@RBAH. l Representative CLSM images of immunostaining (HIF-1α, CD31) in 4T1 tumors after different treatments. Green, HIF-1α; red, CD31; blue, DAPI. Scale bar, 100 μm. m Representative γ-H2Aχ fluorescence staining images of 4T1 tumors after different treatments. Green, γ-H2Aχ; blue, DAPI. Scale bar, 200 μm. Experiments in ( a , j , k , l , m ) were independently repeated three times with comparable results. Data in ( c – k ) were presented as mean ± SD. n = 3 mice per group. Statistical significance was determined using two-way ANOVA ( c ) and two-sided unpaired student’s t -test ( d , e , g , j , k ). Source data are provided as a Source Data file.

Article Snippet: Horseradish peroxidase (HRP) (Cat# P8020), BCA protein assay kit (Cat# PC0020), live/dead cell double stain kit (Cat #CA1630), and red blood cell lysis buffer (Cat #R1010) were purchased from Beijing Solarbio Science & Technology Co., Ltd. Recombinant Mouse IL-12 (Cat# CM39) was purchased from Novoprotein.

Techniques: In Vivo, Fluorescence, Imaging, Injection, Saline, Immunostaining, Staining